{"id":19327,"date":"2021-11-22T10:13:24","date_gmt":"2021-11-22T09:13:24","guid":{"rendered":"https:\/\/adlbionatur.com\/?page_id=19327"},"modified":"2022-09-28T11:55:00","modified_gmt":"2022-09-28T09:55:00","slug":"click-bio","status":"publish","type":"page","link":"https:\/\/biatgroup.com\/es\/tecnologias\/click-bio\/","title":{"rendered":"CLICK BIO"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"19327\" class=\"elementor elementor-19327 elementor-19326\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-313bd31 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"313bd31\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-901fb02\" data-id=\"901fb02\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e5e10ae elementor-widget elementor-widget-spacer\" data-id=\"e5e10ae\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-column .elementor-spacer-inner{height:var(--spacer-size)}.e-con{--container-widget-width:100%}.e-con-inner>.elementor-widget-spacer,.e-con>.elementor-widget-spacer{width:var(--container-widget-width,var(--spacer-size));--align-self:var(--container-widget-align-self,initial);--flex-shrink:0}.e-con-inner>.elementor-widget-spacer>.elementor-widget-container,.e-con-inner>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer,.e-con>.elementor-widget-spacer>.elementor-widget-container,.e-con>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer{height:100%}.e-con-inner>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer>.elementor-spacer-inner,.e-con>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer>.elementor-spacer-inner{height:var(--container-widget-height,var(--spacer-size))}<\/style>\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-525355e elementor-widget elementor-widget-heading\" data-id=\"525355e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">Why SPLITTERA? CLICK BIO<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2475ce elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"b2475ce\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#2c2c2c;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:\"\";border-bottom:0;flex-grow:1;border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right .elementor-divider__element{margin-right:0}.elementor-widget-divider:not(.elementor-widget-divider--view-line_text):not(.elementor-widget-divider--view-line_icon) .elementor-divider-separator{border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--separator-type-pattern{--divider-border-style:none}.elementor-widget-divider--separator-type-pattern.elementor-widget-divider--view-line .elementor-divider-separator,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b658066 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"b658066\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-extended\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-72dc8f9\" data-id=\"72dc8f9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8b83e4d elementor-widget elementor-widget-text-editor\" data-id=\"8b83e4d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>Based on its <b>specific reaction<\/b>, SPLITTERA is a&nbsp;<b>simple<\/b>, <b>fast, efficient and cost-effective universal&nbsp;<\/b><b>tool for CLICK-BIO<\/b>, with multiple applications. It&nbsp;creates a <b>new covalent bond <\/b>between the protein&nbsp;of interest and the payload, <b><i>in vitro <\/i><\/b><b>or <\/b><b><i>in vivo&nbsp;<\/i><\/b><b>without modifying the origina<\/b>l protein sequence&nbsp;or properties.<\/p>\r\n<p><b>SOME USES <\/b><\/p>\r\n<ul>\r\n<li><b>Pharma product:<\/b>\r\n<ul>\r\n<li>ADC (Antibody Drug Conjugate)<\/li>\r\n<li>Companion Ab<\/li>\r\n<li>Therapeutic fusion proteins<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><b>Immuno assays <\/b><\/li>\r\n<li><b>Assay development <\/b><\/li>\r\n<li><b>Screenings<\/b><\/li>\r\n<\/ul>\r\n<p>One of the main applications is customization of antibodies, allowing the creation of antibodies libraries functionalized on demand, a technology of great value for antibody-based clinical programs in immunotherapy.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-a31364c\" data-id=\"a31364c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1467079 elementor-widget elementor-widget-heading\" data-id=\"1467079\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Key Advantages<\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0167c74 elementor-widget elementor-widget-text-editor\" data-id=\"0167c74\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li><strong>VERSATILITY:<\/strong><ul><li><strong>Any recombinant protein:<\/strong> No limit in type, size or structure.<\/li><li><strong>Any Payload:<\/strong> cytotoxin, tag, fluorescent probe, peptide, DNA, chemical compound, Ab portions&#8230;<\/li><li><strong>Family of 4 orthogonal split-inteins:<\/strong> Don\u2019t cross react, thus one single protein can be specifically modified with more than one different payload.<\/li><\/ul><\/li><li>Split-inteins are not present in the final product (scar of max 6 amino acids)<ul><li>No immunogenic, suitable for in-vivo applications.<\/li><li>Conjugated proteins show the same properties as the unconjugated (antigen specificity, internalization)<\/li><\/ul><\/li><li>Intein fusions can be produced in any expression host, without affecting expression and solubility.<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e0b83c9 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"e0b83c9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c9e7932\" data-id=\"c9e7932\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-92dee77 elementor-widget elementor-widget-spacer\" data-id=\"92dee77\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e030897 elementor-widget elementor-widget-heading\" data-id=\"e030897\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Properties<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e082d92 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"e082d92\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-cfc601d elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"cfc601d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-06510d7\" data-id=\"06510d7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-69ea02f elementor-widget elementor-widget-text-editor\" data-id=\"69ea02f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li><b>4 different <\/b>orthogonal pairs of inteins.<\/li><li>N-ints: <b>80aa<\/b>, C-Ints: <b>40 aa<\/b>.<\/li><li>Extremely <b>FAST<\/b>, high reaction <b>YIELD. <\/b><\/li><li>100% <b>SPECIFICITY<\/b>, no interaction with other split inteins<\/li><li>Minimal amino acid requirement.<\/li><li>Active under a wide range of conditions (pH 6-9\u00a0and 4-45\u00b0C).<\/li><li>Accepting detergents and chaotropic agents<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-dbe8e82\" data-id=\"dbe8e82\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-588b970 elementor-widget elementor-widget-image\" data-id=\"588b970\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"478\" height=\"461\" src=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-2.jpg\" class=\"attachment-full size-full wp-image-19423\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-2.jpg 478w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-2-300x289.jpg 300w\" sizes=\"(max-width: 478px) 100vw, 478px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Figure 2. Extremely fast ligation reaction reaching completion after only 30 minutes<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-273830e elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"273830e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b9b7cb0\" data-id=\"b9b7cb0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ed30dee elementor-widget elementor-widget-spacer\" data-id=\"ed30dee\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b4d5df elementor-position-left elementor-view-default elementor-mobile-position-top elementor-vertical-align-top elementor-widget elementor-widget-icon-box\" data-id=\"7b4d5df\" data-element_type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<link rel=\"stylesheet\" href=\"https:\/\/biatgroup.com\/wp-content\/plugins\/elementor\/assets\/css\/widget-icon-box.min.css\">\t\t<div class=\"elementor-icon-box-wrapper\">\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span class=\"elementor-icon elementor-animation-\" >\n\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-long-arrow-alt-right\"><\/i>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t<span  >\n\t\t\t\t\t\tMultilabelling\t\t\t\t\t<\/span>\n\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aa52242 elementor-widget elementor-widget-text-editor\" data-id=\"aa52242\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>4 orthogonal split-inteins to incorporate different payloads on the same protein, conferring extra added value.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9fd1d9d elementor-widget elementor-widget-image\" data-id=\"9fd1d9d\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1255\" height=\"491\" src=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-3.jpg\" class=\"attachment-full size-full wp-image-19425\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-3.jpg 1255w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-3-300x117.jpg 300w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-3-1024x401.jpg 1024w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-3-768x300.jpg 768w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-3-700x274.jpg 700w\" sizes=\"(max-width: 1255px) 100vw, 1255px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-44562b6 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"44562b6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4995be0\" data-id=\"4995be0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e7bb03d elementor-widget elementor-widget-spacer\" data-id=\"e7bb03d\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cde376c elementor-position-left elementor-view-default elementor-mobile-position-top elementor-vertical-align-top elementor-widget elementor-widget-icon-box\" data-id=\"cde376c\" data-element_type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span class=\"elementor-icon elementor-animation-\" >\n\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-long-arrow-alt-right\"><\/i>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t<span  >\n\t\t\t\t\t\tBioconjugates: site-specific modification of proteins\t\t\t\t\t<\/span>\n\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fedb41a elementor-widget elementor-widget-text-editor\" data-id=\"fedb41a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Conjugation of any protein (<b>Ab, Fabs, ScFv, nanobodies, bispecifics<\/b>,etc.) with the desired payload (<b>toxin,\u00a0<\/b><b>fluorescent or colorimetric probes<\/b>,\u00a0<b>chemical compound<\/b>, <b>DNA, peptides, proteins<\/b>, <b>IgG derivates) <\/b>to\u00a0generate the final product for a given application.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-25bb882 elementor-widget elementor-widget-image\" data-id=\"25bb882\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1152\" height=\"441\" src=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-4.jpg\" class=\"attachment-full size-full wp-image-19427\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-4.jpg 1152w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-4-300x115.jpg 300w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-4-1024x392.jpg 1024w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-4-768x294.jpg 768w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-4-700x268.jpg 700w\" sizes=\"(max-width: 1152px) 100vw, 1152px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d482584 elementor-widget elementor-widget-text-editor\" data-id=\"d482584\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li><b>Homogeneous<\/b>, <b>site-specifically <\/b>modified protein<\/li><li><b>No modification or mutation <\/b>on functional domain of mAb<\/li><li><b>Controllable DAR <\/b>(Drug to Antibody Ratio) <b>2, 4, 2+2<\/b>, different payloads<\/li><li><b>No need <\/b>for non-natural amino acids or sugars, enzymes or cofactors<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-395e30c elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"395e30c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9385337\" data-id=\"9385337\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9f8ee67 elementor-widget elementor-widget-spacer\" data-id=\"9f8ee67\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6875bcc elementor-position-left elementor-view-default elementor-mobile-position-top elementor-vertical-align-top elementor-widget elementor-widget-icon-box\" data-id=\"6875bcc\" data-element_type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span class=\"elementor-icon elementor-animation-\" >\n\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-long-arrow-alt-right\"><\/i>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t<span  >\n\t\t\t\t\t\tBispecifics: Ab formats and Fc-Fusions\t\t\t\t\t<\/span>\n\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2452f5a elementor-widget elementor-widget-text-editor\" data-id=\"2452f5a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The orthogonality of Splittera split-inteins allows different strategies to obtain bispecifics, other IgG\u00a0formats as well as Fc-Fusions.<\/p><p>Using a different pair of Splittera split-inteins, these molecules can also be labelled with any payload.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8763979 elementor-widget elementor-widget-image\" data-id=\"8763979\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"1068\" height=\"406\" src=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-5.jpg\" class=\"attachment-full size-full wp-image-19429\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-5.jpg 1068w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-5-300x114.jpg 300w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-5-1024x389.jpg 1024w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-5-768x292.jpg 768w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/tecnologia-5-700x266.jpg 700w\" sizes=\"(max-width: 1068px) 100vw, 1068px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ed90889 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"ed90889\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e9a4356\" data-id=\"e9a4356\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3a22287 elementor-widget elementor-widget-spacer\" data-id=\"3a22287\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7ae8e35 elementor-position-left elementor-view-default elementor-mobile-position-top elementor-vertical-align-top elementor-widget elementor-widget-icon-box\" data-id=\"7ae8e35\" data-element_type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\t\t\t\t\t\t<div class=\"elementor-icon-box-icon\">\n\t\t\t\t<span class=\"elementor-icon elementor-animation-\" >\n\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-long-arrow-alt-right\"><\/i>\t\t\t\t<\/span>\n\t\t\t<\/div>\n\t\t\t\t\t\t<div class=\"elementor-icon-box-content\">\n\t\t\t\t<h3 class=\"elementor-icon-box-title\">\n\t\t\t\t\t<span  >\n\t\t\t\t\t\tIn vivo and in vitro\t\t\t\t\t<\/span>\n\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6cbe625 elementor-widget elementor-widget-text-editor\" data-id=\"6cbe625\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>SPLITTERA split inteins are not present in the final product, leaving a scar of maximum 6 aminoacids.\u00a0<\/p><p>Consequently, conjugated products show the same properties (antigen specificity, internalization,\u00a0etc) as the original, and are not immunogenic, making it possible to use SPLITTERA for in <i>in-vivo <\/i>as\u00a0well as in <i>in-vitro <\/i>applications.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2610166 elementor-widget elementor-widget-image\" data-id=\"2610166\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img width=\"878\" height=\"418\" src=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/bio-6.jpg\" class=\"attachment-full size-full wp-image-19431\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/bio-6.jpg 878w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/bio-6-300x143.jpg 300w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/bio-6-768x366.jpg 768w, https:\/\/biatgroup.com\/wp-content\/uploads\/2021\/11\/bio-6-700x333.jpg 700w\" sizes=\"(max-width: 878px) 100vw, 878px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Figure 3. Antigen specificity comparison between a non-modified IgG (mAb, blue line) and the same IgG with 2 toxin molecules fused to the HC (mAb-tox, green line)<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Why SPLITTERA? CLICK BIO Based on its specific reaction, SPLITTERA is a&nbsp;simple, fast, efficient and cost-effective universal&nbsp;tool for CLICK-BIO, with multiple applications. It&nbsp;creates a new covalent bond between the protein&nbsp;of interest and the payload, in vitro or in vivo&nbsp;without modifying the original protein sequence&nbsp;or properties&#8230;.<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":19539,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false},"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/pages\/19327"}],"collection":[{"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/comments?post=19327"}],"version-history":[{"count":2,"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/pages\/19327\/revisions"}],"predecessor-version":[{"id":21336,"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/pages\/19327\/revisions\/21336"}],"up":[{"embeddable":true,"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/pages\/19539"}],"wp:attachment":[{"href":"https:\/\/biatgroup.com\/es\/wp-json\/wp\/v2\/media?parent=19327"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}