CLICK’N CUT™ is a universal and robust tool to obtain tag-less proteins in a one-step process based on intein affinity. It is suitable for every step of product and process development: R&D, pre-clinical, clinical and industrial manufacturing.
HOW DOES CLICK’N CUT™ WORK?
POI recombinantly produced in the host of choice fused to IntC.
Resin, with IntN covalently linked to it, captures IntC-POI fusion thanks to the high affinity existing between the two intein domains.
All undesired proteins are washed away.
Intein mediated cleavage is triggered by a change in buffer’s pH.
Pure tag-less protein elutes from the column
The column can be easily regenerated.
KEY FEATURES OF CLICK'N CUT™
Universal system to purify any POI
High purity (>95%)
Fast (5 hours to pure, tag-less POI)
Simple: cleavage is triggered on column by a pH shift at physiological conditions
Compatible with common expression host and buffersa pH shift at physiological conditions.
Generates a clean cut with no extra residues
No premature cleavage during protein expression
Cleavage takes place regardless of the identity of the first POI’s amino acid