Biobide CRO — Aquatics Testing Services
Biobide — The Premier Zebrafish CRO
Accelerating R&D with Aquatic NAMs. High-fidelity in-vivo data. In-vitro speed. Zero regulatory animal burden.
Why Zebrafish NAMs
In-vivo data quality. In-vitro speed.
Zebrafish embryo models deliver whole-organism biological relevance with the scalability and speed of in-vitro methods. Under EU Directive 2024/1262, assays conducted on embryos prior to 120 hpf are classified as NAMs — zero animal welfare regulatory burden.
High Genetic Homology
Over 80% genetic similarity with humans. Systemic biological context unavailable in purely cellular models.
Speed & Throughput
Results in 2-4 weeks. High-throughput ready with standard microplate compatibility. 100-300 eggs per couple/week.
GLP Certified
Fully GLP-certified environment (ENAC 2026). Results accepted by regulatory agencies for preclinical studies.
Services by Industry
Tailored Solutions for Every Sector
Agrochemical · Chemical · Cosmetic · Environmental · Food Ingredients
Comprehensive aquatic models testing portfolio for regulatory compliance and de-risk R&D across chemical, cosmetic and environmental sectors. GLP and non-GLP options available.
Freshwater Alga Growth Inhibition.
Daphnia sp. Acute Immobilisation Test.
Fish Acute Toxicity Test.
Fish Early-Life Stage Toxicity Test.
Daphnia magna Reproduction Test.
Fish Embryo Acute Toxicity Test.
Fish Cell Line Acute Toxicity: RTgill-W1 Assay. non-GLP.
OECD-recognized level 3 mechanistic assays for ED assessment.
Thyroid hormone disruptors.
Estrogenic ER agonist screening. Safety by Design.
Androgenic and antiandrogenic activity.
Estrogenic and antiestrogenic activity.
Thyroid gland disruptors.
Developmental and Reproductive Toxicity screening. EC50, LC50, Teratogenic Index, NOAEL.
Heart rate, arrhythmia and fibrillation via proprietary Cardio software.
Hepatic opacity, steatosis (Oil-Red), histopathology. Lipid metabolism.
DNT: locomotor and photomotor responses during CNS organogenesis.
Leukocyte infiltration (innate) and lymphopoiesis (adaptive).
Morphological changes in kidney development.
DASPEI staining to visualize neuromasts after 24h treatment.
Acute toxicity in fish embryos (FET).
Specialized kits. KIE, AOPs, metabolic pathways, epigenetic effects.
HPLC-MS/MS: ingredient quantification in the embryo matrix.
H&E, Oil-Red, Toluidine Blue in 18+ organs: liver, intestine, brain.
Germ-free zebrafish. Selective colonization with strains of interest.
Diet-induced obesity: NFD and HFD. BMI, triglycerides, glycemia.
Shoaling, exploration, anxiety. Functional readout for gut-brain axis.
Pharma & Biotech
Full preclinical safety and efficacy portfolio. All assays available except GLP ecotoxicity. Custom disease models and target validation.
DART screening. EC50, LC50, Teratogenic Index, NOAEL.
Acute toxicity in fish embryos (FET).
Heart rate, arrhythmia and fibrillation. Proprietary Cardio software.
Acute opacity, steatosis and histopathology.
Morphological changes in kidney development.
Developmental Neurotoxicity (DNT). DanioVision system.
Innate and adaptive immune system tracking.
DASPEI staining. Neuromast visualization after 24h treatment.
EASZY, RADAR, REACTIV, XETA, ZETA. OECD-recognized assays.
ALS, Dravet Syndrome, Parkinson's, Duchenne Muscular Dystrophy, Tauopathy.
Fast, statistically significant, high repeatability evaluation of specific targets.
Diet-induced obesity: NFD and HFD. BMI, triglycerides, glycemia.
KIE, AOPs, metabolic pathways, epigenetic effects.
Shoaling, exploration, anxiety. Gut-brain axis functional readout.
HPLC-MS/MS: ingredient quantification in the embryo matrix.
