SPLITTERA is a simple, fast, efficient and cost effective universal tool that allows conjugation of VLPs with antigens of any size. It creates a new covalent bond between the structural proteins of the VLP and the antigens of interest, leading to the functionalization of VLPs by antigen display. SPLITTERA improves the possibilities of classical VLPs overcoming the current limits in antigen size. Moreover, it is compatible with antigens produced in any host.
SPLITTERA split inteins are not present in the final product, leaving a scar of maximum 6 aminoacids. Consequently, VLPs display antigens maintaining their properties, making it possible to use SPLITTERA to conjugate antigens of any size.