CLICK´N CUT
CLICK’N CUT™ FOR TAG-LESS PROTEIN PURIFICATION
Now
We offer a free sample of our Click’N Cut Kit with a pre-packed 1 mL column and the necessary buffers for you to try it out.
CLICK’N CUT™ is a universal and robust tool to obtain tag-less proteins in a one-step process based on intein affinity. It is suitable for every step of product and process development: R&D, pre-clinical, clinical and industrial manufacturing.
HOW DOES CLICK’N CUT™ WORK?
- POI recombinantly produced in the host of choice fused to IntC.
- Resin, with IntN covalently linked to it, captures IntC-POI fusion thanks to the high affinity existing between the two intein domains.
- All undesired proteins are washed away.
- Intein mediated cleavage is triggered by a change in buffer’s pH.
- Pure tag-less protein elutes from the column
- The column can be easily regenerated.
KEY FEATURES OF CLICK'N CUT™
- Universal system to purify any POI
- High purity (>95%)
- Fast (5 hours to pure, tag-less POI)
- Simple: cleavage is triggered on column by a pH shift at physiological conditions
- Compatible with common expression host and buffersa pH shift at physiological conditions.
- Generates a clean cut with no extra residues
- No premature cleavage during protein expression
- Cleavage takes place regardless of the identity of the first POI’s amino acid
