Based on its specific reaction, SPLITTERA is a universal tool that allows to efficiently immobilize any recombinant protein in a controlled, homogenous and convenient orientation, resulting in diagnostic tests showing higher detection sensitivity.
C-Int is covalently attached to a solid support, using a unique Cys or other chemical methods. Any ligand can be produced in fusion to Int-N. The specific intein trans-splicing reaction between C-Int and N-Int allows to covalently immobilize the ligand into the solid support by a unique standardized reaction. Sensitivity is increased as a consequence of the right orientation of all ligand molecules. The sensitivity is increased by 50% when using SPLITTERA, allowing to detect lower levels of the target protein.