Based on its specific reaction, SPLITTERA is a simple, fast, efficient and cost-effective universal tool for CLICK-BIO, with multiple applications. It creates a new covalent bond between the protein of interest and the payload, in vitro or in vivo without modifying the original protein sequence or properties.
SOME USES
One of the main applications is customization of antibodies, allowing the creation of antibodies libraries functionalized on demand, a technology of great value for antibody-based clinical programs in immunotherapy.
4 orthogonal split-inteins to incorporate different payloads on the same protein, conferring extra added value.
Conjugation of any protein (Ab, Fabs, ScFv, nanobodies, bispecifics,etc.) with the desired payload (toxin, fluorescent or colorimetric probes, chemical compound, DNA, peptides, proteins, IgG derivates) to generate the final product for a given application.
The orthogonality of Splittera split-inteins allows different strategies to obtain bispecifics, other IgG formats as well as Fc-Fusions.
Using a different pair of Splittera split-inteins, these molecules can also be labelled with any payload.
SPLITTERA split inteins are not present in the final product, leaving a scar of maximum 6 aminoacids.
Consequently, conjugated products show the same properties (antigen specificity, internalization, etc) as the original, and are not immunogenic, making it possible to use SPLITTERA for in in-vivo as well as in in-vitro applications.
