Biobide CRO — Aquatics Testing Services
Biobide · CRO Services
The full testing catalogue
GLP-certified, OECD-aligned zebrafish and ecotoxicology testing across pharmaceutical, agrochemical, industrial chemical, cosmetic and food industries. Every guideline below links directly from the industry pages — jump to any assay with its own address.
Ecotoxicology & Environmental Risk Assessment
GLP-ready ecotoxicology modules for standard ERA packages and higher-tier refinements — from algae to avian models, aligned with OECD and ISO guidelines.
Aquatic Models
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 201 | Freshwater Alga, Growth Inhibition Test | Growth inhibition |
| OECD TG 202 | Daphnia sp. Acute Immobilisation Test | Acute immobilisation |
| OECD TG 211 | Daphnia magna Reproduction Test | Chronic reproduction |
| ISO 20665 | Ceriodaphnia dubia Chronic Toxicity | Chronic toxicity |
| OECD TG 203 | Fish Acute Toxicity Test | Acute toxicity |
| OECD TG 210 | Fish Early-Life Stage Toxicity | Early-life stage |
| OECD TG 236 | Fish Embryo Acute Toxicity (FET) Test | Acute toxicity (embryo, NAM) |
| OECD TG 249 | RTgill-W1 Fish Cell Line Acute Toxicity | In vitro cell-line toxicity |
Sediment & Benthic Invertebrates
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 218 | Sediment-Water Chironomid Toxicity, Spiked Sediment | Toxicity |
| OECD TG 219 | Sediment-Water Chironomid Toxicity, Spiked Water | Toxicity |
| OECD TG 233 | Chironomid Life-Cycle Toxicity Test | Life-cycle toxicity |
| OECD TG 235 | Chironomus sp. Acute Immobilisation Test | Acute immobilisation |
| OECD TG 225 | Sediment-Water Lumbriculus Toxicity Test | Toxicity |
| OECD TG 242 | Potamopyrgus antipodarum Reproduction Test | Reproduction |
| OECD TG 243 | Lymnaea stagnalis Reproduction Test | Reproduction |
Aquatic Macrophytes
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 221 | Lemna sp. Growth Inhibition Test | Growth inhibition |
| OECD TG 238 | Sediment-Free Myriophyllum spicatum Test | Growth |
| OECD TG 239 | Water-Sediment Myriophyllum spicatum Test | Growth |
Terrestrial Invertebrates & Soil
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 207 | Earthworm, Acute Toxicity Test | Acute toxicity |
| OECD TG 222 | Earthworm Reproduction Test | Reproduction |
| ISO 11268-1/2 | Earthworm Acute & Reproduction Tests | Acute / reproduction |
| OECD TG 220 | Enchytraeid Reproduction Test | Reproduction |
| OECD TG 232 | Collembolan Reproduction Test in Soil | Reproduction |
| OECD TG 226 | Predatory Mite Reproduction Test in Soil | Reproduction |
| OECD TG 208 | Seedling Emergence & Seedling Growth Test | Emergence / growth |
| OECD TG 227 | Terrestrial Plant Vegetative Vigour Test | Vegetative vigour |
| OECD TG 216 | Soil Microorganisms: Nitrogen Transformation | Nitrogen transformation |
| OECD TG 217 | Soil Microorganisms: Carbon Transformation | Carbon transformation |
Pollinators & Non-Target Arthropods
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 213 | Honeybees, Acute Oral Toxicity Test | Acute oral |
| OECD TG 214 | Honeybees, Acute Contact Toxicity Test | Acute contact |
| OECD TG 245 | Honey Bee Chronic Oral Toxicity Test | Chronic oral |
| OECD TG 237 | Honey Bee Larval Toxicity, Single Exposure | Larval toxicity |
| OECD GD 239 | Honey Bee Larval Toxicity, Repeated Exposure | Larval toxicity |
| OECD GD 75 | Honey Bee Brood Test under Semi-Field Conditions | Brood development |
| OECD TG 246 | Bumblebee, Acute Contact Toxicity Test | Acute contact |
| OECD TG 247 | Bumblebee, Acute Oral Toxicity Test | Acute oral |
| IOBC/WPRS | Non-Target Arthropods: Tier I & Extended Lab | Tier I / extended lab |
| EPPO 170 | Beneficial Arthropod Standard Test Methods | Standard methods |
Higher-Tier Vertebrates
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 229 | Fish Short Term Reproduction Assay | Reproduction |
| OECD TG 230 | 21-Day Fish Assay | Endocrine screening |
| OECD TG 234 | Fish Sexual Development Test | Sexual development |
| OECD TG 240 | Extended One-Generation Reproduction Test | Multi-generation reproduction |
| OECD TG 231 | Amphibian Metamorphosis Assay | Metamorphosis |
| OECD TG 241 | Larval Amphibian Growth and Development Assay | Growth & development |
| OECD TG 223 | Avian Acute Oral Toxicity Test | Acute oral |
| OECD TG 205 | Avian Dietary Toxicity Test | Dietary toxicity |
| OECD TG 206 | Avian Reproduction Test | Reproduction |
Microbial (WWTP-relevant)
| Guideline | Test | Endpoint |
|---|---|---|
| OECD TG 209 | Activated Sludge Respiration Inhibition | Respiration inhibition |
| OECD TG 224 | Anaerobic Bacteria Activity Inhibition | Activity inhibition |
| OECD TG 244 | Protozoan Activated Sludge Inhibition | Activity inhibition |
Systemic & Developmental Toxicology — Safety Assays on Zebrafish
Most assays run on zebrafish embryos (<5 dpf), classified as NAMs (New Approach Methodologies). Applicable to pharmaceutical, biotech, cosmetic and nutraceutical pipelines for early non-clinical toxicity profiling.
| Assay | Endpoint measured | Method |
|---|---|---|
| ACUTETOX | LC50, EC50 | OECD 236-based. 4 lethal endpoints: coagulation, somites, heartbeat, tail detachment. 2 & 4 dpf assessment. |
| TERATOTOX | EC50, LC50, TI, NOAEL | Zebrafish Embryotoxicity Test (ZET). 10+ morphological endpoints. Teratogenic Index enables DART prioritization. |
| CARDIOTOX | Heart rate, arrhythmia | GFP-heart transgenic line. Proprietary Cardio v3.0 software. Fibrillation, bradycardia, AV-block detection. |
| HEPATOTOX | Opacity, steatosis, area | GFP-liver line. Acute opacity, fluorescent intensity, lipid vacuoles (oil-red), histopathology. RT-PCR add-on. |
| NEUROTOX | 10+ locomotor parameters | DanioVision (Noldus). Light-dark transition test. Velocity, distance, duration, frequency. DNT endpoint. |
| OTOTOX | Neuromast count | DASPEI vital staining. Lateral-line hair-cell damage after 24h exposure. Ototoxicity & protective agent screening. |
| IMMUNOTOX | Leukocyte infiltration | Fluorescent immune-cell lines. Innate (leukocyte quantification) + adaptive (lymphopoiesis) endpoints. |
| NEPHROTOX | Pronephros structure | Kidney morphology (pronephros). Clearance functional assay. Nephroprotection screening. |
Level 3 Endocrine Disruption Screening
OECD-recognized in-vivo NAM fluorescent readouts. Unmatched sensitivity for endocrine MIE (Molecular Initiating Event) validation and regulatory compliance.
| Assay | Guideline | Mechanism & readout |
|---|---|---|
| EASZY | OECD TG 250 (GLP) | Cyp19a1b-GFP transgenic zebrafish; fluorescent brain readout for estrogenic receptor activity. Validated at 100% accuracy, sensitivity and specificity. |
| ZETA | Screening-tier (GLP) | Tg(tg:mCherry) transgenic zebrafish; thyroglobulin promoter reporter reflects thyroid follicle activity. |
Genotoxicity — Core In Vitro & In Vivo Follow-Up
GLP-ready genotoxicity modules for regulatory packages: core in vitro battery plus triggered in vivo follow-up.
Core In Vitro
| Guideline | Test |
|---|---|
| OECD TG 471 | Bacterial Reverse Mutation (Ames) Test |
| OECD TG 487 | In Vitro Mammalian Cell Micronucleus Test |
| OECD TG 476 / 490 | In Vitro Mammalian Cell Gene Mutation Tests |
| OECD TG 473 | In Vitro Mammalian Chromosomal Aberration Test |
| — | Mini-Ames / Fluctuation Ames Screening |
In Vivo Follow-Up
| Guideline | Test |
|---|---|
| OECD TG 474 | Mammalian Erythrocyte Micronucleus Test |
| OECD TG 475 | Bone Marrow Chromosome Aberration Test |
| OECD TG 489 | In Vivo Mammalian Alkaline Comet Assay |
| OECD TG 488 | Transgenic Rodent Gene Mutation Assay |
| OECD TG 486 | Liver UDS Assay |
| OECD TG 483/478/485/477 | Germ-Cell & Heritable Assays |
| ICH S2(R1) | Weight-of-Evidence Follow-Up Strategy |
Efficacy Assays & Disease Model Generation
Beyond safety — Biobide's platform supports target validation, compound efficacy screening and custom disease model generation for therapeutic discovery.
Efficacy Screening Capabilities
| Capability | Description |
|---|---|
| Target Validation | Fast, statistically robust, high-repeatability evaluation of specific molecular targets in a live whole-organism context |
| High-Content Screening (HCS) | Automation + image analysis + ML/AI for in-vivo phenotypic screening. Transgenic reporter lines |
| Bioavailability Confirmation | LC-MS/MS internal concentration in embryo matrix to rule out false negatives |
| Transcriptomics / AOPs | Full RNA-seq and RT-qPCR panels. Key Initiating Events (KIE) and Adverse Outcome Pathway (AOP) ready |
Disease Model Generation
| Therapeutic Area | Models |
|---|---|
| CNS / Neurodegenerative | Duchenne Muscular Dystrophy · ALS · Dravet Syndrome · Tauopathy |
| Chemically Induced | Epilepsy · Parkinson's disease |
| Oncology | Xenograft models for tumor growth & angiogenesis |
| Cardiovascular | Arrhythmia models · Cardiac channel screening |
| Metabolism | Hepatic steatosis · Metabolic disease models |
Environmental Fate & Behaviour
Environmental fate & behaviour modules for ERA packages: core screening plus triggered/refinement studies.
Sorption & Mobility
| Guideline | Test |
|---|---|
| OECD TG 106 | Adsorption / Desorption Using Batch Equilibrium |
| OECD TG 121 | Estimation of Adsorption Coefficient (Koc) |
| OECD TG 312 | Leaching in Soil Columns |
| OECD TG 107/117 | Partition Coefficient / Log Kow Inputs |
Abiotic Transformation
| Guideline | Test |
|---|---|
| OECD TG 111 | Hydrolysis as a Function of pH |
| OECD TG 316 | Phototransformation in Water: Direct Photolysis |
| FOCUS / SETAC | Photolysis & Hydrolysis Kinetic Evaluation |
| — | Transformation Product ID & Mass Balance (TP Profiling) |
Biodegradation Screening
| Guideline | Test |
|---|---|
| OECD TG 301 | Ready Biodegradability |
| OECD TG 310 | Ready Biodegradability: CO2 Headspace Test |
| OECD TG 302 A/B/C | Inherent Biodegradability Screening |
| OECD TG 306/311 | Seawater & Anaerobic Biodegradation |
Wastewater & STP Fate
| Guideline | Test |
|---|---|
| OECD TG 303A | Activated Sludge Units Simulation |
| OECD TG 303B | Biofilms Simulation Test |
| OECD TG 314B | Activated Sludge Biodegradation Simulation |
| ISO 11733 | Activated Sludge Simulation Test |
Soil, Water & Sediment Transformation
| Guideline | Test |
|---|---|
| OECD TG 307 | Aerobic / Anaerobic Transformation in Soil |
| OECD TG 308 | Aquatic Sediment Transformation Systems |
| OECD TG 309 | Surface Water Simulation Biodegradation |
| FOCUS / SETAC | Degradation Kinetics & DT50 Modelling |
Bioaccumulation & Metabolism
| Guideline | Test |
|---|---|
| OECD TG 305 | Bioaccumulation in Fish |
| OECD TG 315 | Bioaccumulation in Sediment Oligochaetes |
| OECD TG 317 | Bioaccumulation in Terrestrial Oligochaetes |
| OECD TG 319 A/B | In Vitro Fish Hepatic Clearance Assays |
Technology Platform — Automation, AI & High-Content Screening
The infrastructure and software behind every assay: automated imaging, proprietary analysis pipelines and a 100,000-fish facility.
| Capability | Description |
|---|---|
| High-Content Screening (HCS) | Automated image acquisition and analysis of zebrafish embryos, algae and Daphnia. ML and AI-driven phenotypic readouts for high-throughput compound profiling |
| Proprietary Software | Biobide Cardio v3.0 for heart rate analysis. DanioVision (Noldus) for neurobehavioral tracking. Custom fluorescence quantification pipelines for ED assays |
| Transgenic Zebrafish Library | 70+ strains: GFP-heart, GFP-liver, Cyp19a1b-GFP (EASZY), Tg(tg:mCherry) thyroid, immune-cell reporters and disease-model lines. MTA-sourced or in-house generated |
| Multi-Omics Add-Ons | RT-qPCR target panels. Full transcriptomics (RNA-seq) with KIE/AOP annotation. T3/T4 whole-embryo quantification by LC-HRMS. Bioavailability (LC-MS/MS, GC-MS, UV-Vis) |
| Histopathology | Whole-body and organ-specific sectioning. H&E, toluidine blue, oil-red staining. Liver, brain, eye, thyroid, spinal cord. Available in larvae and adults |
| 100,000+ Fish Facility | In-house zebrafish facility in San Sebastián. Controlled access, highest welfare standards. Algae and Daphnia cultures maintained in parallel for ecotox testing |
GLP Quality, Reproducibility & Regulatory Track Record
GLP-Certified
Fully compliant with OECD Principles of GLP for ecotox assays.
Regulatory Collaboration
Active collaboration with EPA, FDA, NIH/NIEHS, and OECD on assay validation and acceptance.
Industry-Leading Clients
BASF, Corteva, DSM-Firmenich, L'Oréal, NIH, Afton Chemical, MMV, DNDi, among others.
Scientific Publication Record
20+ peer-reviewed publications supporting assay validity, cross-lab reproducibility and regulatory acceptance.
- Prioritizing chemicals for developmental neurotoxicity by integrating data from a new approach methods (NAMs) battery covering key cellular events in neurodevelopment. Neurotoxicology 115 (2026) 103485.
- Zebrafish behavioral assessment as a first-tier whole-organism NAM for developmental neurotoxicity: A multi-laboratory evaluation. Neurotoxicology 116 (2026) 103504.
- Zebrafish embryotoxicity test (ZET) as an alternative method for screening the developmental toxicity of difficult-to-test substances. Reproductive Toxicology 142 (2026) 109224.
De-Risk Your Compounds
Share your compound list and context of use — we'll propose a tailored assay battery, screening through GLP regulatory, with a dedicated Study Director from day one.
clientes@biobide.com
+34 943 309 360
